Phage integrase



Phage integrase (PIN) mediate recombination between DNA recognition sequences - the phage attP and the bacterial attB. The Tyrosine family PIN uses a catalytic tyrosine to mediate strand cleavage while the Serine family PIN uses a serine residue. The High-Pathogenicity Island (HPI) is a genomic island essential for virulence of the family of Enterobacteria.

3D Structures of phage integrase
Update June 2011

3nrw – PIN/site-specific recombinase N-terminal – Haloarcula marismortui

3jtz – PIN arm-type binding domain – Yersinia pestis

3ju0 – HPI PIN arm-type binding domain – Pectobacterium atrosepticum

2kkp – PIN SAM-like domain – Moorella thermoacetica – NMR

2kkv – PIN fragment – Salmonella enterica – NMR

2khq - PIN fragment – Staphylococcus saprophyticus – NMR

2wcc – λPIN DBD+ DNA – Enterobacteria phage λ - NMR

1kjk – λPIN N-terminal - NMR

2oxo, 1z19, 1z1b - λPIN core binding domain + DNA

1ae9 - λPIN catalytic core domain (mutant)

1z1g – λPIN + DNA (Holliday junction)

1p7d – λPIN fragment + DNA

3bvp – PIN catalytic core domain – Lactococcus phage TP101-1

1aih - PIN catalytic core domain – Haemophilus phage HP1

2khv, 2kj5 – PIN residues 102-199 – Nitrosospira multiformis - NMR